![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K3476012
Designed by: Daria Shumkova Group: iGEM20_MSP-Maastricht (2020-10-19)
shRNA Wg_b (Thaumetopoea processionea)
Part is encoding a short hairpin RNA against the Wg-gene in Oak Processionary Catterpillars(Thaumetopoea processionea). Made for restriction cloning with EcoR1 & BamH1 into vector L4440.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal BamHI site found at 54 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/basic_part
//function/degradation
//function/degradation
None |